CpG methylation as a tool to characterize cell-free Kaposi sarcoma herpesvirus DNA.
نویسندگان
چکیده
We studied the presence of Kaposi sarcoma herpesvirus sequences in cell-free DNA (cfDNA) isolated from the blood of patients with AIDS-related Kaposi sarcoma (KS) and primary effusion lymphoma (PEL). The use of paramagnetic beads linked to methyl-CpG binding domain protein allowed separation of virion and cell-derived DNA. Only virion DNA was detected in the blood of KS patients, whereas cell-derived DNA was detected in a patient with AIDS-related PEL. The difference in the origins of cfDNA in these settings may in part reflect very different proliferative indices in KS and PEL tumor tissue.
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عنوان ژورنال:
- The Journal of infectious diseases
دوره 205 7 شماره
صفحات -
تاریخ انتشار 2012